Optimum Condition for Polyhydroxyalkanoate Production from Crude Glycerol by Bacillus sp. Isolated from Lipid-Containing Wastewater

Polyhydroxyalkanoates (PHAs) are a group of biopolymers used as an alternative to petroleum-based synthetic plastics. Their industrial application is not widely available due production cost constraints, mainly from raw materials for carbon sources. This research focuses on selecting Bacillus strain lipid-containing wastewater that can produce PHAs crude glycerol and investigating the optimum conditions producing glycerol. The was isolated storage pond at fermented pork sausage manufacturing plant in Thailand utilizing Mineral Salt Medium (MSM) with 1 % w/v glucose source. synthesis preliminarily investigated by staining cells Sudan Black B. FMI3 produced highest 43 dry cell weight (DCW). It identified pumilus FMI3. Crude obtained by-product transesterification waste cooking oil catalysed NaOH. partially purified acidification neutralization exhibited about 80 w/w glycerol, 1.90 ± 0.09 ash content, 2.80 0.14 water light brown colour, pH 7.11 0.35. Isolated grows best produces yields under conditions, i.e., MSM containing ammonium sulphate 61:1 mole ratio nitrogen sources respectively, 15 inoculum, initial neutral 7, shaking 150 rpm 40 °C 72 h. These achieved 79 DCW, classified medium-chain-length comprising 3-hydroxyoctanoate,3-hydroxydecanoate, 3-hydroxydodecanoate, 3-hydroxytetradecanoate 9, 12, 67 12 %, respectively. study reported feasibility feedstock strain, which able grow tolerate salts HIGHLIGHTS Bacteria endure extreme environments (i.e., very limited nutrients; drought chemicals) polyhydroxyalkanoates presented yield achieve condition GRAPHICAL ABSTRACT